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- Title
How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics.
- Authors
Silvestri, Teresa; Immirzi, Barbara; Dal Poggetto, Giovanni; Di Donato, Paola; Mollo, Valentina; Mayol, Laura; Biondi, Marco
- Abstract
Polymeric microparticles (MPs) designed for the intravitreal administration of therapeutic proteins result in a prolonged half-life in the vitreous and can delay or discourage the onset of adverse effects inevitably related to this route of administration. Hence, here we designed MPs composed of a polymeric blend based on poly(lactic-co-glycolic) acid and poloxamers, externally decorated with hyaluronic acid. The MPs are intended for intravitreal administration of bovine serum albumin. In detail, a systematic formulative study aiming to shed light on the complex relationship between protein release rate and MP degradation rate was carried out by means of calorimetric and gel permeation chromatography analyses. We found out that poloxamer addition caused a compact MP matrix, which led to a slight modification of the degradation kinetics and a reduction in the initial BSA initial release, which is of the utmost importance to ensure a relatively regular BSA release. It must also be underlined that for acid-labile molecules such as proteins, the poloxamer's presence induced complex and hardly predictable effects on MP degradation/protein release, due to the dynamic balance between the time-evolving hydrophilicity of MPs and the influence of poloxamers themselves on the PLGA degradation rate.
- Subjects
POLYMER degradation; PROTEOLYSIS; POLOXAMERS; DYNAMIC balance (Mechanics); SERUM albumin
- Publication
Applied Sciences (2076-3417), 2021, Vol 11, Issue 16, p7567
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app11167567