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- Title
The Ki67+ proliferation index correlates with increased cellular retinol-binding protein-1 and the coordinated loss of plakophilin-1 and desmoplakin during progression of cervical squamous lesions.
- Authors
Schmitt-Graeff, A; Koeninger, A; Olschewski, M; Haxelmans, S; Nitschke, R; Bochaton-Piallat, M-L; Lifschitz-Mercer, B; Gabbiani, G; Langbein, L; Czernobilsky, B
- Abstract
Aims: To investigate the modulation of cellular retinol-binding protein (CRBP)-1 and the desmosomal plaque proteins plakophilin (PKP)-1 and desmoplakin (DP) in correlation with the Ki67+ proliferation index (PI) during the progression of cervical squamous intraepithelial lesions (SIL) to squamous cell carcinoma (SCC). Methods: Using in situ imaging by brightfield and confocal laser scanning microscopy, the expression of CRBP-1 protein and transcripts, PKP-1, DP and the Ki67 PI were analysed in 38 low-grade (L) SIL, 56 high-grade (H) SIL, 49 SCC, 30 control cervices and 10 human papillomavirus-positive condylomatous lesions. Results: CRBP-1+ cells increased from 11.4% in the normal cervix to 80.3% in LSILs, 92.3% in HSILs and slightly decreased to 78.3% in invasive SCCs ( P = 0.0001) in close association with the Ki67 PI ( r =0.41; P < 0.0001). PKP-1+ and DP+ cells were correlated (0.32; P < 0.0001) and decreased from normal (81% versus 92.3%) to LSIL (53.1% versus 85.3%), to HSIL (46.4% versus 67.5%) and SCC (35.1% versus 35.9%). The Ki67+ PI was inversely correlated with DP (−0.20, P = 0.0014) and PKP-1 (−0.19, P = 0.015). Condylomata retained low CRBP-1 and high expression of PKP-1 and DP. Conclusions: The gain of CRBP-1 and the loss of desmosomal proteins occur early in cervical carcinogenesis.
- Subjects
CARRIER proteins; VITAMIN A; SQUAMOUS cell carcinoma; PAPILLOMAVIRUSES; DESMOSOMES; MEDICAL research
- Publication
Histopathology, 2007, Vol 51, Issue 1, p87
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2007.02724.x