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- Title
Expression of transmembrane protein tyrosine phosphatase gamma (PTPγ) in normal and neoplastic human tissues.
- Authors
Vezzalini, M.; Mombello, A.; Menestrina, F.; Mafficini, A.; Della Peruta, M.; van Niekerk, C.; Barbareschi, M.; Scarpa, A.; Sorio, C.
- Abstract
Aims: To establish the conditions for protein tyrosine phosphatase gamma (PTPγ) detection in paraffin tissues using two antibodies raised against its NH2- (anti-P4) and COOH-termini (γTL1); to analyse its expression in normal tissues and to perform an initial screening of neoplastic tissues. Methods and results: Membranous and/or cytoplasmic PTPγ expression was detected in the majority of epithelial cell types and in endocrine cells, with the highest expression in adrenal medulla, endocrine cells of the gastrointestinal tract and pancreatic islets. Both antibodies stained the thyroid follicular epithelium, but only anti-P4 antibody stained the colloid matrix, suggesting shedding/secretion of the PTPγ extracellular domain. Marked loss of PTPγ immunoreactivity was detected in subsets of ovarian (21%), breast (56%) and lung (80%) neoplasms. Conversely, cytoplasmic positivity was found in 37% of lymphomas, mainly of high-grade histotypes, while normal lymphocytes were negative. Brain tissue showed PTPγ expression in a few neuronal and glial elements and PTPγ was overexpressed in the majority of high-grade astrocytomas. Conclusions: We have analysed PTPγ expression in archival paraffin-embedded tissues for the first time, demonstrating particularly high expression in endocrine cells and both down- and up-regulation in neoplasia, the latter possibly reflecting the undifferentiated state of the neoplastic cells, suggesting a complex role for this phosphatase.
- Subjects
PROTEIN-tyrosine phosphatase; TISSUES; TUMORS; IMMUNOGLOBULINS; PATHOLOGY
- Publication
Histopathology, 2007, Vol 50, Issue 5, p615
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2007.02661.x