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- Title
Intracellular Survival of Staphylococcus aureus in Endothelial Cells: A Matter of Growth or Persistence.
- Authors
Rollin, Guillaume; Xin Tan; Tros, Fabiola; Dupuis, Marion; Nassif, Xavier; Charbit, Alain; Coureuil, Mathieu
- Abstract
The Gram-positive human pathogen Staphylococcus aureus is a leading cause of severe bacterial infections. Recent studies have shown that various cell types could readily internalize S. aureus and infected cells have been proposed to serve as vehicle for the systemic dissemination of the pathogen. Here we focused on the intracellular behavior of the Community-Associated Methicillin-Resistant S. aureus strain USA300. Supporting earlier observations, we found that wild-type S. aureus strain USA300 persisted for longer period within endothelial cells than within macrophages and that a mutant displaying the small colony variant phenotype (1hemDBL) had increased intracellular persistence. Time-lapse microscopy revealed that initial persistence of wildtype bacteria in endothelial cells corresponded to distinct single cell events, ranging from active intracellular bacterial proliferation, leading to cell lysis, to non-replicating bacterial persistence even 1 week after infection. In sharp contrast, ΔhemDBL mutant bacteria were essentially non-replicating up to 10 days after infection. These findings suggest that internalization of S. aureus in endothelial cells triggers its persistence and support the notion that endothelial cellsmight constitute an intracellular persistence niche responsible for reported relapse of infection after antibiotic therapy.
- Subjects
METHICILLIN-resistant staphylococcus aureus; ENDOTHELIAL cells; MACROPHAGES
- Publication
Frontiers in Microbiology, 2017, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2017.01354