We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induction of esterase activity during the degradation of high concentrations of the contaminant di(2-ethylhexyl) phthalate by Fusarium culmorum under liquid fermentation conditions.
- Authors
González-Márquez, Angel; Loera-Corral, Octavio; Viniegra-González, Gustavo; Sánchez, Carmen
- Abstract
In this study, the induction of esterase activity during the degradation of a high concentration of di(2-ethylhexyl) phthalate (DEHP) (1500 mg l−1) by Fusarium culmorum was investigated using Ca(NO3)2 as nitrogen source under liquid fermentation conditions. Assessments of esterase activities through biochemical tests and zymographic assays, as well as fungal growth were studied. A high concentration of DEHP increased esterase activity in F. culmorum, which produces five esterase isoforms (26.4, 31.7, 43, 73.6 and 125 kDa), which were different in abundance and molecular weight to those produced constitutively in glucose-containing medium (control medium). F. culmorum showed higher µ and YX/S values in DEHP-containing medium than those observed in the control medium. F. culmorum has great potential for use in the restoration of sites contaminated with high concentrations of DEHP and even of other phthalates with less complex structures.
- Subjects
FUSARIUM culmorum; PHTHALATE esters; FERMENTATION; FUNGAL growth; MOLECULAR weights
- Publication
3 Biotech, 2020, Vol 10, Issue 11, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-020-02476-y