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- Title
S-nitrosylation of the zinc finger protein SRG1 regulates plant immunity.
- Authors
Cui, Beimi; Pan, Qiaona; Clarke, David; Villarreal, Marisol Ochoa; Umbreen, Saima; Yuan, Bo; Shan, Weixing; Jiang, Jihong; Loake, Gary J.
- Abstract
Nitric oxide (NO) orchestrates a plethora of incongruent plant immune responses, including the reprograming of global gene expression. However, the cognate molecular mechanisms remain largely unknown. Here we show a zinc finger transcription factor (ZF-TF), SRG1, is a central target of NO bioactivity during plant immunity, where it functions as a positive regulator. NO accumulation promotes SRG1 expression and subsequently SRG1 occupies a repeated canonical sequence within target promoters. An EAR domain enables SRG1 to recruit the corepressor TOPLESS, suppressing target gene expression. Sustained NO synthesis drives SRG1 S-nitrosylation predominantly at Cys87, relieving both SRG1 DNA binding and transcriptional repression activity. Accordingly, mutation of Cys87 compromises NO-mediated control of SRG1-dependent transcriptional suppression. Thus, the SRG1-SNO formation may contribute to a negative feedback loop that attenuates the plant immune response. SRG1 Cys87 is evolutionary conserved and thus may be a target for redox regulation of ZF-TF function across phylogenetic kingdoms. Upon pathogen infection plants accumulate nitric oxide which subsequently regulates defence gene expression. Here, the authors show that S-nitrosylation of the zinc finger transcription factor SRG1 affects transcriptional suppression and contributes to activation of defence responses.
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-06578-3