We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A novel environment-evoked transcriptional signature predicts reactivity in single dentate granule neurons.
- Authors
Jaeger, Baptiste N.; Linker, Sara B.; Parylak, Sarah L.; Barron, Jerika J.; Gallina, Iryna S.; Saavedra, Christian D.; Fitzpatrick, Conor; Lim, Christina K.; Schafer, Simon T.; Lacar, Benjamin; Jessberger, Sebastian; Gage, Fred H.
- Abstract
Activity-induced remodeling of neuronal circuits is critical for memory formation. This process relies in part on transcription, but neither the rate of activity nor baseline transcription is equal across neuronal cell types. In this study, we isolated mouse hippocampal populations with different activity levels and used single nucleus RNA-seq to compare their transcriptional responses to activation. One hour after novel environment exposure, sparsely active dentate granule (DG) neurons had a much stronger transcriptional response compared to more highly active CA1 pyramidal cells and vasoactive intestinal polypeptide (VIP) interneurons. Activity continued to impact transcription in DG neurons up to 5 h, with increased heterogeneity. By re-exposing the mice to the same environment, we identified a unique transcriptional signature that selects DG neurons for reactivation upon re-exposure to the same environment. These results link transcriptional heterogeneity to functional heterogeneity and identify a transcriptional correlate of memory encoding in individual DG neurons. Single nuclei RNA-seq has been used to characterize transcriptional signature of environment-related activity in cells of the dentate gyrus. Here the authors use this approach to show that whether a neuron will be reactivated in response to re-exposure to a previous environment can be predicted by its transcriptional signature.
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-05418-8