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- Title
Whole genome analysis to the molecular characterization of enterohemorrhagic Escherichia coli O157:H7 Outbreak in kindergarten, Gyeonggi-do, 2020.
- Authors
Wooju-Kang; Jungsun Park; Eunkyung Shin; Seung-Eun Song; Jaeil Yoo; Junyoung Kim
- Abstract
배경 Enterohemorragic Escherichia coli (EHEC) is the representative pathogenic bacteria inducing diarrhea, and especially hemolytic uremic syndrome (HUS). We aimed to investigate the molecular characterization of EHEC O157:H7 outbreak in kindergarten of Gyeonggi-do, June 2020. 방법 Forty-five EHEC isolates were collected from 110 patients and characterized using multiplex toxin PCR assays and O-serotyping. Antimicrobial susceptibility testing was performed using broth microdilution method. The genetic relationships were determined using pulsed-field gel electrophoresis (PFGE) by di- gestion with XbaI. Furthermore, based on PFGE pattern analysis, selected isolates were analyzed by whole genome sequencing (WGS) and whole genome multilocus sequence typing (wgMLST). 결과 The isolates were identified as EHEC O157:H7 and resistant to ampicillin. The PFGE results showed four different XbaI-PFGE patterns (EHHX01.205(n=41), EHHX01.206(n=2), EHHX01.207(n=1), EHHX01.210(n=1)), but these patterns were highly related. The wgMLST analysis showed that 12 EHEC O157:H7 isolates (EHHX01.205) were closely related the each other genetically (range = 0-1 alleles differences) and the suggesting that the EHEC O157:H7 found in patients all shared a common source. Unfortunately, we hadn’t to found a source of EHEC contamination in the outbreak. 결론 In this study, the molecular epidemiology of the outbreak was investigated using WGS analysis of EHEC isolates. As WGS becomes more integrated into foodborne outbreak detection, public health researchers will need to learn about the technology and its interpretation.
- Subjects
ESCHERICHIA coli O157:H7; WHOLE genome sequencing; PULSED-field gel electrophoresis; HEMOLYTIC-uremic syndrome; MICROBIAL sensitivity tests; MOLECULAR virology; EXOTOXIN
- Publication
Infection & Chemotherapy, 2022, Vol 54, pS267
- ISSN
2093-2340
- Publication type
Article