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- Title
Alleles of the Yeast PMS1 Mismatch-Repair Gene That Differentially Affect Recombinations and Replication-Related Processes.
- Authors
Welz-Voegele, Caroline; Stone, Jana E.; Tran, Phuoc T.; Kearney, Hutton M.; Liskay, R. Michael; Petes, Thomas D.; Jinks-Robertson, Sue
- Abstract
Mismatch-repair (MMR) systems promote eukaryotic genome stability by removing errors introduced during DNA replication and by inhibiting recombination between nonidentical sequences (spellchecker and antirecombination activities, respectively). Following a common mismatch-recognition step effected by MutS-homologous Msh proteins, homologs of the bacterial MutL ATPase (predominantly the Mlh1pPms1p heterodimer in yeast) couple mismatch recognition to the appropriate downstream processing steps. To examine whether the processing steps in the spellchecker and antirecombination pathways might differ, we mutagenized the yeast PMS1 gene and screened for mitotic separation-of-function alleles. Two alleles affecting only the antirecombination function of Pms1p were identified, one of which changed an amino acid within the highly conserved ATPase domain. To more specifically address the role of ATP binding/hydrolysis in MMR-related processes, we examined mutations known to compromise the ATPase activity of Pmslp or Mlh1p with respect to the mitotic spellchecker and antirecombination activities and with respect to the repair of mismatches present in meiotic recombination intermediates. The results of these analyses confirm a differential requirement for the Prms1p ATPase activity in replication vs. recombination processes, while demonstrating that the Mlhlp ATPase activity is important for all examined MMRrelated functions.
- Subjects
DNA replication; NUCLEOTIDE sequence; PMS genes
- Publication
Genetics, 2002, Vol 162, Issue 3, p1131
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1093/genetics/162.3.1131