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- Title
O DESAFIO PARA COMPREENDER A MORFOLOGIA CEREBRAL: DA VISUALIZAÇÃO À QUANTIFICAÇÃO.
- Authors
Regina de Melo, Silvana; de Oliveira, Rúbia Maria Monteiro W.; Velasquez de Oliviera, Daniela; Caroline Bernardi, Tuanay; Milani, Humberto
- Abstract
Neuronal morphology is analyzed in animal models to provide biological information difficult to obtain in humans. The above makes possible the study of neuro-psychiatric, such as Alzheimer's disease, anxiety and others. Current study described methodology for rodents' brains, two neuro-anatomic techniques, Klüver-Barrera and Golgi-Cox, and their respective quantification processes. Klüver-Barrera technique visualized the white and gray matter, particularly the myelin sheath. Golgi-Cox technique, adapted for current research, was efficient to visualize neurons and their prolongations, such as dendrites and dendritic spines, with quantification. Images by microscope described the different steps for the quantification, determination of volume of the brain's internal structures (callous body and the hypocampus 's cell layer) coupled to the quantification of dendritic spines in pyramid neurons. Described and detailed methods will be useful in several fields of neuroscience.
- Publication
Saúde e Pesquisa, 2019, Vol 12, Issue 1, p97
- ISSN
1983-1870
- Publication type
Article
- DOI
10.17765/2176-9206.2019v12n1p97-106