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- Title
COMPARANDO EL EFECTO ANTIMICROBIANO DE JUGOS Y PULPAS DE FRUTAS INDUSTRIALES PRODUCIDOS EN PERÚ, FRENTE A BACTERIAS PATÓGENAS. ESTUDIO PRELIMINAR.
- Authors
RAMOS GUERRERO, Félix Giovani; SANCHEZ RODRIGUEZ, Pablo Daniel; NOA BARRIENTOS, Luis Adolfo; RAMOS GORBEÑA, Juan Carlos; AGURTO SÁENZ, Tomás
- Abstract
Background: Due to food safety issues, the industry of fruit juices and pulps apply treatments capable of reducing the pertinent pathogen by 5-log, however the most of these treatments do not take into account the antimicrobial properties of each fruit. Objectives: The main aim of this study was to compare the antimicrobial effect of industrialized fruit juices and pulps manufactured at Callao (Peru) against pathogenic bacteria, by using agar well diffusion method. Methods: Groups of Muller Hinton agar plates were prepared and inoculated with 108 CFU/ml of Listeria monocytogenes ATCC 19145, Escherichia coli O157:H7 OPS 191, Salmonella enterica subsp enterica ATCC 14028 and Staphylococcus aureus ATCC 6538. Each plate was circularly perforated in 6 equidistant points. Wells were filled with 50 μl of Passion Fruit Juice (PFJ), Camu Camu Pulp (CCP), Lulo Pulp (LP), Mango Criollo Pulp (McP), Cherimoya Pulp (ChP) and Strawberry Pulp (SP) according to the case, and the antimicrobial activity was measured through of diameter (mm) in the zone of inhibition at 24 hours. Results: McP, ChP and SP showed no any antimicrobial effect against the microorganisms evaluated (0.0 mm), while that LP does not have antimicrobial activity against S. enterica subsp enterica and St. aureus. PFJ and CCP did not showed significant differences in the effect of inhibition against L. monocytogenes, followed by LP (13.16 mm). E. coli O157:H7 was affected by PFJ, CCP and LP showing an average inhibition zone of 14.81, 13.73 y 12.17 mm respectively, while that St. aureus was inhibited only by CCP (12.30 mm) and PFJ (11.07 mm). CCP had great antimicrobial effect against S. enterica subsp enterica (14.30 mm) in comparison to PFJ (11.99 mm). Conclusions: PFJ and CCP showed high antimicrobial activity against pathogenic bacteria. This should be used by industries, in order to apply less severe heat treatments, without neglecting the quality and safety of them.
- Publication
Vitae (01214004), 2016, Vol 23, pS71
- ISSN
0121-4004
- Publication type
Article