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- Title
Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas.
- Authors
Xuan, Ming; Fang, Yi‐Ru; Wato, Masahiro; Hata, Shintaro; Tanaka, Akio
- Abstract
Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC,n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral.J Oral Pathol Med(2005)34: 334–9
- Subjects
ANGIOMAS; BLOOD-vessel tumors; SQUAMOUS cell carcinoma; ORAL mucosa; IMMUNOGLOBULINS; MONOCLONAL antibodies; DIAGNOSTIC immunohistochemistry
- Publication
Journal of Oral Pathology & Medicine, 2005, Vol 34, Issue 6, p334
- ISSN
0904-2512
- Publication type
Article
- DOI
10.1111/j.1600-0714.2005.00316.x