We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of genetic aberrations in cell lines from oral squamous cell carcinomas by comparative genomic hybridization.
- Authors
Okafuji, Masaki; Ita, Masamichi; Hayatsu, Yoshikazu; Shinozaki, Fumihiko; Oga, Atsunori; Sasaki, Kohsuke; Okafuji, M; Ita, M; Hayatsu, Y; Shinozaki, F; Oga, A; Sasaki, K
- Abstract
We detected genetic alterations in 14 cell lines established from 14 human oral squamous cell carcinomas (OSCCs) using comparative genomic hybridization (CGH), which allows a comprehensive analysis of chromosomal imbalances and identification of nonrandom genetic aberrations specific to OSCCs. All cell lines showed gains and losses of DNA copy number. DNA losses were detected for chromosomes 18q (10/14) and 4q (9/14) with minimal overlapping regions of 18q12-32 and 4q31-qter, respectively. In contrast, the common sites for increased copy number were chromosomes 5p (12/14), 8q23-ter (11/14), 20p (8/14), 20q (8/14), and 3q25-ter (7/14). These results suggest that losses of 18q12-22 and 4q31-ter and gains of 5p and 8q23-ter play important roles in the development and/or progression of OSCC.
- Subjects
CHROMOSOME abnormalities; CANCER cells; COMPARATIVE genomic hybridization; DNA; CELL lines; PATHOLOGY; ANEUPLOIDY; CHROMOSOMES; COMPARATIVE studies; FLOW cytometry; DIGITAL image processing; RESEARCH methodology; MEDICAL cooperation; MOUTH tumors; NUCLEIC acid hybridization; RESEARCH; SQUAMOUS cell carcinoma; EVALUATION research; CANCER cell culture; GENOTYPES
- Publication
Journal of Oral Pathology & Medicine, 1999, Vol 28, Issue 6, p241
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1999.tb02032.x