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- Title
大蒜素对脑胶质瘤细胞U87细胞侵袭能力的影响及其机制.
- Authors
蔡青; 秦怀洲; 陈昆仑
- Abstract
Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms.Methods In this study,we employed MTT assay to test the anti-proliferative effect of allicin.Transwell assay was used to test the anti-metastatic ability of allicin.Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9).Western blotting was employed to test the phosphorylated level of p38.ResultsAllicin could significantly inhibit the proliferation and invasion of U87 cells(concentration>8μg/mL,P<0.05).Meanwhile allicin(concentration<8μg/mL)could inhibit the invasion of U87 cells.After treatment with allicin for24 hours,the expressions of MMP-2 and MMP-9 were decreased significantly(P <0.05).Moreover,allicin treatment decreased the phosphorylated level of p38 obviously(P <0.05).Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway,suggesting that allicin is a potential therapeutic agent for glioma.
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2015, Vol 36, Issue 2, p271
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201502024