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- Title
Identification and Genetic Evolution Analysis of One Strain of H3N2 Canine Influenza Virus Isolated from Nanjing, China.
- Authors
Kalhoro, Dildar Hussain; Shan Liang; Kalhoro, Muhammad Saleem; Pirzado, Shoaib Ahmed; Rajput, Nasir; Naeem, Muhammad; Parveen, Fahmida; Yongjie Liu
- Abstract
Nasopharyngeal swabs were collected from 6 dogs with severe respiratory syndrome at the Animal Clinics of Nanjing Agricultural University in 2014. One viral strain was isolated from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
- Subjects
GENETIC mutation; INFLUENZA A virus, H3N2 subtype; ANIMAL welfare; INFLUENZA viruses; AMINO acids
- Publication
Pakistan Journal of Zoology, 2018, Vol 50, Issue 3, p817
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2018.50.3.817.824