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- Title
Characterization of Cellulose Degrading Bacterium, Bacillus megaterium S3, Isolated from Indigenous Environment.
- Authors
Shakoor, Shumaila; Aftab, Saira; Rehman, Abdul
- Abstract
A cellulose degrading bacterium, identified as Bacillus megaterium S3 on the basis of biochemical and 16S rRNA ribotyping, was isolated from vegetable market, Lahore, Pakistan. It was screened by efficiently growing on carboxymethyl cellulose (CMC-Na), Congo red agar, and minimal salt medium containing filter paper. The most suitable temperature for the growth of B. megaterium S3 was found to be 45°C while the optimum growth pH was 7. B. megaterium S3 had a lag phase of 4 hours in LB medium while this phase prolonged to 6 hours in CMC-Na medium. B. megaterium S3 had shown maximum enzyme activity in extra-cellular assay (75%) as compared to intra-cellular assay (17%). The optimum temperature and pH for the crude enzyme activity were found to be 50°C and 8, respectively. Maximum crude enzyme extract (cellulase) activity was found in the presence of Zn ions. This alkali-thermophilic enzyme from B. megaterium S3 can be exploited for biotechnological and industrial applications.
- Subjects
BACILLUS megaterium; CARBOXYMETHYLCELLULOSE; CONGO red (Staining dye); BACTERIAL growth; HYDROGEN-ion concentration; BACTERIAL enzymes
- Publication
Pakistan Journal of Zoology, 2013, Vol 45, Issue 6, p1655
- ISSN
0030-9923
- Publication type
Article