We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Quantification of Galectin-7 and Its Localization in Adult Mouse Tissues1.
- Authors
Sato, Miki; Nishi, Nozomu; Shoji, Hiroki; Kumagai, Mika; Imaizumi, Tadaatsu; Hata, Yuiro; Hirashima, Mitsuomi; Suzuki, Shigehiko; Nakamura, Takanori
- Abstract
We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 ± 3 ng/mg; esophagus, 23 ± 8 ng/mg; stomach, 18 ± 6 ng/mg; anus, 13 ± 1 ng/mg; and tongue, 12 ± 2 ng/mg. This indicates that galectin-7 production coincides with the degree of stratification of the epithelia. Interestingly, we also detected significant amounts of galectin-7, 5.9 ± 1.4 and 2.7 ± 0.6 ng/mg, in the trachea and ovaries, respectively. Moreover, we found that galectin-7 is localized in the pseudostratified epithelium of the trachea and stromal epithelium of the ovaries by immunohistochemistry. Thus, galectin-7 protein might be produced primarily in stratified epithelia, but also in some wet epithelia, and plays a unique role in cell-mucus contact, or the growth of ovarian follicles.
- Subjects
GALECTINS; EPITHELIUM; TISSUES; WESTERN immunoblotting; ESOPHAGUS; IMMUNOHISTOCHEMISTRY
- Publication
Journal of Biochemistry, 2002, Vol 131, Issue 2, p255
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a003096