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- Title
Apoptosis induced by β,β-dimethylacrylshikonin is associated with Bcl-2 and NF-κB in human breast carcinoma MCF-7 cells.
- Authors
YAO XIONG; XIU-YING MA; ZIRAN ZHANG; ZHEN-JUN SHAO; YUAN-YUAN ZHANG; LI-MING ZHOU
- Abstract
β,β-dimethylacrylshikonin (DA) is a natural naphthoquinone derivative compound of Lithospermum erythrorhizon with various biological activities. The present study aimed to investigate the inhibitory effects and underlying mechanisms of DA in human breast carcinoma MCF-7 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that DA inhibited the proliferation of MCF-7 cells in a dose- and time-dependent manner. The half maximal inhibitory concentration of DA with regard to the proliferation of MCF-7 cells was 0.050±0.016 mM. The characteristics of cell apoptosis, including cell shrinkage, nuclear pyknosis and chromatin condensation, were all observed in DA-treated cells. DA decreased the expression levels of Bcl-2 and increased the expression of Bax and caspase-3 compared with those in the control. DA inhibited the activity of the nuclear factor (NF)-κB pathway, by downregulating the expression of the p65 subunit, and inhibited the Iκb phosphorylation. DA inhibits the proliferation of MCF-7 cells in vitro by inducing apoptosis through the downregulation of Bcl-2, upregulation of Bax and partial inactivation of the NF-κB pathway.
- Subjects
BREAST cancer treatment; APOPTOSIS; APOPTOSIS inducing factor; CANCER cells; CELL lines; BCL-2 proteins; NF-kappa B; TRANSCRIPTION factors; THERAPEUTICS
- Publication
Oncology Letters, 2013, Vol 6, Issue 6, p1789
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2013.1613