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- Title
Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion.
- Authors
Li, X.; Yang, S. H.; Yu, X. C.; Jin, Z. X.; Li, W. D.; Li, L.; Li, J.; Li, M. G.
- Abstract
Aims: To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild-type isolate of B. mucilaginosus D4B1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application. Methods and Results: We constructed a phytase secreting expression vector pSP43 with a mini-Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild-type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36–46-fold when compared with the wild-type strain of D4B1. Conclusions: The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity. Significance and Impact of the Study: A transgenic Bacillus strain by the particle bombardment method was constructed.
- Subjects
BACILLUS (Bacteria); PHOSPHORUS; PHYTASES; TRANSPOSONS; MOBILE genetic elements
- Publication
Journal of Applied Microbiology, 2005, Vol 99, Issue 4, p878
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.2005.02683.x