We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Collaborative studies of dose-response curves obtained with different bioassay methods for soil-applied herbicides.
- Authors
Nyffeler, A.; Gerber, H.-R.; Hurle, K.; Pestemer, W.; Schmidt, R. R.
- Abstract
In a collaborative study by 12 laboratories the reproducibility of bioassay techniques was analysed by comparison and statistical treatment of the ED50values (µg herbicide/g soil). Commonly-used bioassay techniques were investigated; two direct seeding methods, a transplanting method and a shoot extension method. The herbicides studied were two photosynthesis inhibitors, atrazine and metribuzin using <em>Lepidium sativum</em> and <em>Brassica rapa</em> as test plants; two germination inhibitors, tri-allate and trifluralin using <em>Avena sativa</em> as test plant. The mean ED50-value of atrazine in the direct seeding method was 0.18 with a variation from 0.12 to 0.29 and 0.12 (0.07-0.68) in the transplanting method. The values of metribuzin were 0.05 (0.02-0.11) and 0.03 (0.01-0.15) respectively. The values for tri-allate were 1.15 (0.50-2.60) in the direct seeding method and 1.10 (0.54-2.53) in the shoot extension method and for trifluralin 3.11 (0.72-18.31) and 1.33 (0.48-2.94) respectively. The reproducibility was best in the shoot extension method. No outliers (confidence intervals lying entirely outside the confidence interval the overall mean) in the ED50-values were observed with atrazine and metribuzin when using the direct seeding method, whereas with the germination inhibitors a high number of results (four with tri-allate, six with trifluralin) could be considered as outliers. A comparison of the use of fresh weight and dry matter showed good agreement between the ED50-values they gave, with similar reproducibility.
- Subjects
BIOLOGICAL assay; HERBICIDES; PESTICIDES; PHOTOSYNTHESIS; BOTANY
- Publication
Weed Research, 1982, Vol 22, Issue 4, p213
- ISSN
0043-1737
- Publication type
Article
- DOI
10.1111/j.1365-3180.1982.tb00166.x