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- Title
Diversity and distribution of the prokaryotic community in near-surface permafrost sediments in the Tianshan Mountains, China.
- Authors
Daqun Yang; Jianhui Wang; Yu Bai; Shijian Xu; Lizhe An
- Abstract
The community structures and diversity of bacteria and archaea were investigated at 4 depths (1.5, 2.0, 2.5, and 3.0 m) in permafrost sediments in the Tianshan Mountains, using denaturing gradient gel electrophoresis of 16S rRNA gene amplified by polymerase chain reaction. Phylogenetic analysis of the dominant bands sequenced revealed the presence of rich diversity of bacteria, which could be related to the Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Bacteroidetes, Firmicutes, and Chloroflexi. The Proteobacteria, consisting of the α, β, γ, and ε subdivisions, were clearly the dominant group at all depths studied. Archaeal diversity was relatively low and archaeal 16S rRNA gene sequences were grouped into 3 phylogenetic clusters within the 2 kingdoms Euryarchaeota and Crenarchaeota. Within the Euryarchaeota, methanogen-related group II was most abundant at shallow depth (1.5 m), whereas halobacterium-related group I dominated at greater depths. The low-temperature Crenarchaeota group was detected only at 2.5 and 3.0 m. Specific-depth distribution of methanogen-related Euryarchaeota group II and denitrifying bacteria of the genus Pseudomonas dominated at 1.5 m depth, accompanied by a distinct peak in the ratio of NH4-N to NO3/NO2-N, implying the potential capacity of these organisms in near-surface permafrost to release the greenhouse gases N2O and CH4.
- Subjects
FUNGUS-bacterium relationships; BACTERIA; PROKARYOTES; FROZEN ground; ARCHAEBACTERIA; PHASE partition; HALOPHILIC microorganisms; DENITRIFYING bacteria; POLYMERASE chain reaction; GRAM-negative bacteria
- Publication
Canadian Journal of Microbiology, 2008, Vol 54, Issue 4, p270
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W08-004