We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2.
- Authors
Fan Lei; Zhang Dao-Jing; Liu Zhen-Hua; Tao Li-Ming; Luo Yuan-Chan; Wei Hong-Gang; Li Shu-Ian; Li Yuan-Guang
- Abstract
Isolation and purification of antifungal substance from Paenibacillus polymyxa HY96-2, a commercial bioco trol strain used in control of fusarium wilt disease, was studied in this paper. By means of acidification, n-BuOH extraction, ethyl acetate precipitation, silica gel column chromatography, sephadex LH-20 column chromatography and HPLC preparation ,6B was isolated and purified. Its structure was elucidated as fusaricidin A by means of spectroscopic analysis including NMR, MS and ESI-MS/MS. Results of two-fold serial agar dilution method showed that the minimum inhibition concentrations of 6B against 15 phytopathogenic fungi were 12.5 -50 µg/mL. Greenhouse results showed that 6B had better control effect on Botrytis cinerea of cucumber. The control effect could reach 95% when its concentration was set at 250 µg/mL.
- Subjects
ANTIFUNGAL agents; PAENIBACILLUS; HIGH performance liquid chromatography; DILUTION; ETHYL acetate; CHEMICAL structure
- Publication
Natural Product Research & Development, 2012, Vol 24, Issue 6, p729
- ISSN
1001-6880
- Publication type
Article