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- Title
Different effects of Wnt/β-catenin activation and PTH activation in adult and aged male mice metaphyseal fracture healing.
- Authors
Liu, Daocheng; Qin, Hao; Yang, Jiazhi; Yang, Lei; He, Sihao; Chen, Sixu; Bao, Quanwei; Zhao, Yufeng; Zong, Zhaowen
- Abstract
<bold>Background: </bold>Fractures in older men are not uncommon and need to be healed as soon as possible to avoid related complications. Anti-osteoporotic drugs targeting Wnt/β-catenin and PTH (parathyroid hormone) to promote fracture healing have become an important direction in recent years. The study is to observe whether there is a difference in adult and aged situations by activating two signal paths.<bold>Methods: </bold>A single cortical hole with a diameter of 0.6 mm was made in the femoral metaphysis of Catnblox(ex3) mice and wild-type mice. The fracture healing effects of CA (Wnt/β-catenin activation) and PTH (activated by PTH (1-34) injections) were assessed by X-ray and CT imaging on days 7, 14, and 21 after fracture. The mRNA levels of β-catenin, PTH1R(Parathyroid hormone 1 receptor), and RUNX2(Runt-related transcription factor 2) in the fracture defect area were detected using RT-PCR. Angiogenesis and osteoblasts were observed by immunohistochemistry and osteoclasts were observed by TRAP (Tartrate-resistant Acid Phosphatase).<bold>Result: </bold>Adult CA mice and adult PTH mice showed slightly better fracture healing than adult wild-type (WT) mice, but there was no statistical difference. Aged CA mice showed better promotion of angiogenesis and osteoblasts and better fracture healing than aged PTH mice.<bold>Conclusion: </bold>The application of Wnt/β-catenin signaling pathway drugs for fracture healing in elderly patients may bring better early effects than PTH signaling pathway drugs, but the long-term effects need to be observed.
- Subjects
FRACTURE healing; RUNX proteins; ACID phosphatase; OLDER men; MICE
- Publication
BMC Musculoskeletal Disorders, 2020, Vol 21, Issue 1, p1
- ISSN
1471-2474
- Publication type
journal article
- DOI
10.1186/s12891-020-3138-3