We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey.
- Authors
Döşkaya, Mert; Pullukçu, Hüsnü; Karakavuk, Muhammet; Şahar, Esra Atalay; Taşbakan, Mehmet Sezai; Taşbakan, Meltem Işıkgöz; Yılmaz, Mümtaz; Can, Hüseyin; Döşkaya, Aysu Değirmenci; Gürüz, Adnan Yüksel
- Abstract
<bold>Background: </bold>Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect T. gondii RE gene in various human samples by in house and commercial real time polymerase chain reactions.<bold>Methods: </bold>A total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An in house and a commercial RT-PCR were applied to investigate the T. gondii RE gene in these samples.<bold>Results: </bold>The compatibility rate of the two tests was 94.7% (37/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of in house RT-PCR test was 87.5 and 100%. When the in house RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content.<bold>Conclusions: </bold>Both the commercial and in house RT-PCR tests can be used to investigate T. gondii RE gene in various clinical specimens with their high sensitivity and specificity. In house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.
- Subjects
TURKEY; POLYMERASE chain reaction; TOXOPLASMA gondii; AQUEOUS humor; OOCYSTS; HOUSING; THEILERIA; WARM-blooded animals; TRYPANOSOMA cruzi
- Publication
BMC Infectious Diseases, 2019, Vol 19, Issue 1, p1
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/s12879-019-4666-z