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- Title
Simultaneous<sup>1</sup>H- or<sup>2</sup>H-,<sup>15</sup>N- and multiple-band-selective<sup>13</sup>C-decoupling during acquisition in<sup>13</sup>C-detected experiments with proteins and oligonucleotides.
- Authors
Vögeli, Beat; Kovacs, Helena; Pervushin, Konstantin
- Abstract
Significant resolution improvement in13C,13C-TOCSY spectra of uniformly deuterated and13C,15N-labeled protein and13C,15N-labeled RNA samples is achieved by introduction of multiple-band-selective13C-homodecoupling applied simultaneously with1H- or2H- and15N-decoupling at all stages of multidimensional experiments including signal acquisition period. The application of single, double or triple band-selective13C-decoupling in 2D-[13C,13C]-TOCSY experiments during acquisition strongly simplifies the homonuclear splitting pattern. The technical aspects of complex multiple-band homonuclear decoupling and hardware requirements are discussed. The use of this technique (i) facilitates the resonance assignment process as it reduces signal overlap in homonuclear13C-spectra and (ii) possibly improves the signal-to-noise ratio through multiplet collapse. It can be applied in any13C-detected experiment.
- Subjects
OLIGONUCLEOTIDES; PROTEINS; SPECTRUM analysis; RESONANCE; RIBOSE; NUCLEIC acids spectra
- Publication
Journal of Biomolecular NMR, 2005, Vol 31, Issue 1, p1
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1007/s10858-004-5857-4