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- Title
Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus.
- Authors
Shay, Tracy L.; Berghmans, Stéphane; Segers, Karin; Meyers, Stacy; Beever, Jonathan E.; Womack, James E.; Georges, Michel; Charlier, Carole; Cockett, Noelle E.
- Abstract
The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA14q genes. Using Type I loci mapping to the IDVGA30–OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30–OY3 interval. We demonstrate that the IDVGA30– OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.
- Subjects
GENETICS; GENES; LINKAGE (Genetics); GENOMES; GENE mapping; GENOTYPE-environment interaction
- Publication
Mammalian Genome, 2001, Vol 12, Issue 2, p141
- ISSN
0938-8990
- Publication type
Article
- DOI
10.1007/s003350010248