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- Title
An In Vitro Evaluation of the Ability of Ozone Nano- bubble Water to Kill Pathogenic Gram-Negative and Gram-Positive Bacteria.
- Authors
OSMAN, AHMED L.; ALHATTI, REEM; SAWAS, HIBA; ALTOUM, ABD ELGADIR A.; SHADROCH, DEVAPRIYA FINNEY; EL-SAYED, WALID; KANDAKURTIC, PRAVEEN KUMAR; BABKER, ASAAD M. A.
- Abstract
Aim To evaluate the bactericidal effects of ozone Nano-bubble water (NBW3) in different pathogenic bacteria. Methodology 0.1 mL volume of bacteria suspension containing approximately 1 x 107 CFUs/ mL (McFarland standard) of each test bacterium added to each 10 mL of serial dilution of ozone nanobubbles water, 0.9% NaCl with standard strains of bacteria (as negative control) used. Following the incubation (for 2, 5, and 10 min, at 37 °C). The 0.9% NaCl control bacterial suspensions incubated and treated in the same manner. Aliquots removed from each tube at each time point and serial 10-fold dilutions prepared in sterile physiological saline. Next, 0.1 mL aliquots of each dilution aseptically inoculated on plate of agar medium and incubated at 37 °C for overnight. Colonies then be counted, and the number of CFUs/mL be plotted against time for each bacterium tested. Results Ozone had an antibacterial effect on both gram positive and gram-negative bacteria those suspended in first 2 delusion, but little effect in third one. Its antibacterial efficacy was not comparable with that of NaCl under the test conditions used. Ozone-Nano effect on gram negative and gram-positive bacteria. Conclusion Ozone-Nano-bubbles water has bactericidal effect on different strains of gram negative and gram-positive bacteria.
- Subjects
GRAM-positive bacteria; GRAM-negative bacteria; PATHOGENIC bacteria; OZONE; AGAR
- Publication
International Journal of Pharmaceutical Research (09752366), 2020, Vol 12, Issue 3, p2587
- ISSN
0975-2366
- Publication type
Article
- DOI
10.31838/ijpr/2020.12.03.367