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- Title
POTENTIAL EFFECTS OF PLANT ALKALOID BERBERINE ON OVARIAN CELLS IN VITRO.
- Authors
BALDOVSKÁ, S.; MICHALCOVÁ, K.; SANISLO, Ľ.; KOLESÁROVÁ, A.
- Abstract
Berberine (BBR) is a plant isoquinoline alkaloid with a long history in Chinese medicine, present in root and shoot systems of clinically important medicinal plants. This phytochemical compound is found in different plant families such as Berberidaceae, Ranunculaceae and Papaveraceae families. BBR is a widely used as a natural product that showed pharmacological activities in modern and traditional medicine with non-toxic effect on humans. Current clinical research on BBR points out several medical applications, beneficial for therapies of chronic diseases, such as diabetes, hypertension and hypercholesterolemia. However, BBR effects on human reproduction remain unknown. The aim of the in vitro study was to examine the effect of BBR treatment (at the concentrations: 5; 10; 25; 50; 100 µg.ml-1) for 24 h on viability of ovarian cells in vitro. Cultures of human ovarian granulosa-lutein cells (HGL5) and human ovarian carcinoma cells (OVCAR-3) were used, and the metabolic activity was determined by AlamarBlueTM cell viability assay. The viability of HGL5 was not influenced by the berberine treatment at all concentrations used in the study. On the other hand, BBR addition significantly inhibited the viability of OVCAR-3 cells at all doses tested (P = 0.05). In conclusion, our study suggests potential anti-cancer effect of BBR on OVCAR-3 cell line. BBR might be one of such potential agents for ovary cancer therapy. However, further studies are necessary to define a therapeutic potential of BBR.
- Subjects
BERBERINE; ISOQUINOLINE alkaloids; BERBERIDACEAE
- Publication
Slovak Journal of Animal Science, 2018, Vol 51, Issue 4, p174
- ISSN
1337-9984
- Publication type
Abstract