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- Title
Identification of a Unique Germacrene A Oxidase from Xanthium strumarium.
- Authors
Lifen Zheng; Fangfang Chen; Yuanjun Li; Changfu Li
- Abstract
8,12-sesquiterpene lactones (STLs) are an important class of natural products with unique pharmaceutical activities. For years the pathway leading to 8,12-STLs remains an enigma. Xanthium strumarium accumulates abundant 8,12-STLs, and xanthatin is a characteristic 8,12-STL in it. Xanthatin has been previously postulated to be derived from germacrene A, but the steps from germacrene A to xanthatin are unknown. As part of an effort to understand the xanthatin biosynthetic pathway. This study reports the characterization of a unique germacrene A oxidase (XsGAO) from X. strumarium. Unlike a classical GAO enzyme, which is known to catalyze a three-step oxidation of germarene A to yield germacrene A acid (GAA), surprisingly, XsGAO catalyzed only one-step conversion of germacrene A to germacrene A alcohol. We discussed that GAO may be pressured to acquire a novel activity during the evolutionary path for the biosynthesis of 8,12-STLs.
- Subjects
GERMACRENE; XANTHIUM; SESQUITERPENE lactones; BIOSYNTHESIS; PLANT identification
- Publication
Phyton (0031-9457), 2022, Vol 91, Issue 5, p989
- ISSN
0031-9457
- Publication type
Article
- DOI
10.32604/phyton.2022.019219