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- Title
Identification of Cryptic Species of Bemisia tabaci Associated with Tomato in Eastern Dry Zone of Karnataka.
- Authors
SUJATHA, S.; REDDY, K. M. SRINIVAS; REDDY, C. N. LAKSHMINARAYAN; SRINIVASA, N.; SHIVANNA, B.; NAGESH, N.
- Abstract
The whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is an economically important crop pest globally that costs growers billions of dollars to control. The nucleotide sequences of mitochondrial cytochrome oxidase subunit 1 (mtCOI) gene obtained from sample of B. tabaci collected from Tippuru Village, Doddaballapura taluk, Bengaluru Rural (BR) districts of Karnataka was analysed. The total DNA was extracted by Chelex 100 method from single adult whitefly and DNA sample was subjected to PCR using specific primers mtCoIF and mtCOIR for amplifying mtCOI gene, which resulted in the expected PCR amplicon of 800 bp size corresponding to mitochondrial cytochrome oxidase gene sub unit 1. The nucleotide similarity search in the NCBI database confirmed the presence of mtCO1 gene and sequences of cryptic species of B. tabaci showing maximum identity were retrieved for analysis. The mtCOI gene sequence from B. tabaci collected from Doddaballapur showed maximum nucleotide identity of 94.80 per cent with sequences reported from Nepal, which were identified as Asia-II-5 sequences. The findings were well supported by the sequence demarcation graph and phylogenetic analysis. This confirms that whitefly cryptic species collected from Tippuru village, Bengaluru Rural District as B. tabci Asia-II-5 group.
- Subjects
SWEETPOTATO whitefly; BEMISIA; TOMATO diseases &; pests; TOMATO harvesting; VEGETABLE genetics
- Publication
Mysore Journal of Agricultural Sciences, 2021, Vol 55, Issue 4, p349
- ISSN
0047-8539
- Publication type
Article