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- Title
Phytanic Acid Accumulation Is Associated with Conduction Delay and Sudden Cardiac Death in Sterol Carrier Protein-2/Sterol Carrier Protein-x Deficient Mice.
- Authors
MÖNNIG, GEROLD; WIEKOWSKI, JOHANNES; KIRCHHOF, PAULUS; STYPMANN, JÖRG; PLENZ, GABRIELE; FABRITZ, LARISSA; BRUNS, HANS‐JÜRGEN; ECKARDT, LARS; ASSMANN, GERD; HAVERKAMP, WILHELM; BREITHARDT, GÜNTER; SEEDORF, UDO
- Abstract
Phytanic Acid Causes Cardiac Conduction Block. Introduction: The sterol carrier protein-2 gene encodes two functionally distinct proteins: sterol carrier protein-2 (SCP2, a peroxisomal lipid carrier) and sterol carrier protein-x (SCPx, a peroxisomal thiolase known as peroxisomal thiolase-2), which is involved in peroxisomal metabolism of bile acids and branched-chain fatty acids. We show in this study that mice deficient in SCP2 and SCPx (SCP2 null) develop a cardiac phenotype leading to a high sudden cardiac death rate if mice are maintained on diets enriched for phytol (a metabolic precursor of branched-chain fatty acids). Methods and Results: in 210 surface and 305 telemetric ECGs recorded in wild-type (C57BL/6; wt; n = 40) and SCP2 null mice (n = 40), no difference was observed at baseline. However, on diet, cycle lengths were prolonged in SCP2 null mice (262.9 ± 190 vs 146.3±43 msec), AV conduction was prolonged (58.3 ± 17 vs 42.6 ± 4 ms), and QRS complexes were wider (19.1 ± 5 vs 14.0 ± 4 ms). in 11 gene-targeted Langendorff-perfused hearts isolated from SCP2 null mice after dietary challenge, complete AV blocks (n = 5/11) or impaired AV conduction (Wenckebach point 132 ± 27 vs 92 ± 10 msec; P < 0.05) could be confirmed. Monophasic action potentials were not different between the two genotypes. Left ventricular function studied by echocardiography was similar in both strains. Phytanic acid but not pristanic acid accumulated in the phospholipid fraction of myocardial membranes isolated from SCP2 null mice. Conclusion: Accumulation of phytanic acid in myocardial phospholipid membranes is associated with bradycardia and impaired AV nodal and intraventricular impulse conduction, which could provide an explanation for sudden cardiac death in this model.
- Subjects
CARDIAC arrest; STEROLS; HEART conduction system; BRADYCARDIA; FATTY acids; CARDIOLOGY
- Publication
Journal of Cardiovascular Electrophysiology, 2004, Vol 15, Issue 11, p1310
- ISSN
1045-3873
- Publication type
Article
- DOI
10.1046/j.1540-8167.2004.03679.x