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- Title
Anti-HIV-1 Protease Activity of Compounds from Cassia garrettiana.
- Authors
BUNLUEPUECH, Kingkan; TEWTRAKUL, Supinya; WATTANAPIROMSAKUL, Chatchai
- Abstract
For the purpose of discovering anti-HIV-1 agents from Thai plant preparations with a history of use in Thai traditional medicine as agents assisting health longevity, aqueous and ethanol extracts from 24 Thai plants were screened for their inhibitory activities against HIV-1 protease (PR) using an anti-HIV-1 PR assay. Five extracts (10%), 3 ethanol and 2 water, from only 4 plants, Cassia garrettiana, Dioscorea bulbifera, Albizia procera, and Areca catechu, produced extracts with IC50 values of < 45 μg/ml. Thirty five extracts (90%) had no detectable activity, with IC50 values >100 μg/ml. The ethanol extracts of Cassia garrettiana (heartwood), Areca catechu, and Dioscorea bulbifera were the most potent, with IC50 values of 15.6, 24.9, and 44.4 μg/ml, respectively. For the water extracts, the most potent activity was found in Albizia procera (bark) and Cassia garrettiana (heartwood), with IC50 values of 22.6 and 26.8 μg/ml, respectively. Pure compounds from ethanol extracts of Cassia garrettiana (heartwood) were separated to give 5 compounds, chrysophanol (1), piceatannol (2), aloe-emodin (3), emodin (4), and cassigarol E (5). Of the tested samples, piceatannol (2) showed the highest anti-HIV-1 PR activity (IC50 = 48.29 μM), whereas the % inhibition (mean±S.E.M., n=3) produced by 100 μM of cassigarol E (5), aloeemodin (3), chrysophanol (1), and emodin (4) were only 27.64±3.8, 27.20±0.7, 26.66±0.6, and 4.52±1.9, respectively.
- Subjects
CASSIA (Genus); HIV infections; PROTEOLYTIC enzymes; DRUG development; AQUEOUS solutions
- Publication
Walailak Journal of Science & Technology, 2016, Vol 13, Issue 10, p827
- ISSN
1686-3933
- Publication type
Article