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- Title
Growth differentiation factor-15 encodes a novel microRNA 3189 that functions as a potent regulator of cell death.
- Authors
Jones, M F; Ling Li, X; Subramanian, M; Shabalina, Svetlana A; Hara, T; Zhu, Y; Huang, J; Yang, Y; Wakefield, L M; Prasanth, K V; Lal, A
- Abstract
According to the latest version of miRBase, approximately 30% of microRNAs (miRNAs) are unique to primates, but the physiological function of the vast majority remains unknown. In this study, we identified miR-3189 as a novel, p53-regulated, primate-specific miRNA embedded in the intron of the p53-target gene GDF15. Antagonizing miR-3189 increased proliferation and sensitized cells to DNA damage-induced apoptosis, suggesting a tumor suppressor function for endogenous miR-3189. Identification of genome-wide miR-3189 targets revealed that miR-3189 directly inhibits the expression of a large number of genes involved in cell cycle control and cell survival. In addition, miR-3189 downregulated the expression of multiple p53 inhibitors resulting in elevated p53 levels and upregulation of several p53 targets including p21 (CDKN1A), GADD45A and the miR-3189 host gene GDF15, suggesting miR-3189 auto-regulation. Surprisingly, miR-3189 overexpression in p53-/- cells upregulated a subset of p53-targets including GDF15, GADD45A, and NOXA, but not CDKN1A. Consistent with these results, overexpression of miR-3189 potently induced apoptosis and inhibited tumorigenicity in vivo in a p53-independent manner. Collectively, our study identified miR-3189 as a novel, primate-specific miRNA whose effects are mediated by both p53-dependent and p53-independent mechanisms. miR-3189 may, therefore, represent a novel tool that can be utilized therapeutically to induce a potent proapoptotic effect even in p53-deficient tumors.
- Subjects
MICRORNA; CELL death; DNA damage; APOPTOSIS; CELL cycle; TUMORS
- Publication
Cell Death & Differentiation, 2015, Vol 22, Issue 10, p1641
- ISSN
1350-9047
- Publication type
Article
- DOI
10.1038/cdd.2015.9