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- Title
Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg.
- Authors
Ogundajo, Akintayo; Okeleye, Benjamin; Ashafa, Anofi Omotayo
- Abstract
Objectives To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri ( M. barteri ). Methods Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and chemical constituents of the extracts respectively. Results Marked activity was observed in methanol (ME) fraction [MIC 50 : (0.097 7–6.250 0) mg/mL] compared to hexane and ethyl acetate fractions. Aeromonas hydrophila (environmental strain) and Shigella sonnei (ATCC 29930) were the most susceptible pathogens to ME and ciprofloxacin (Cl) at MIC 50 value of 0.097 7 and < 0.019 5 mg/mL respectively. Cryptococcus neoformans (ATCC 66031) was susceptible to ME at 0.195 3 mg/mL compared to fluconazole at 10.000 0 μg/mL. Decreased viability of the Vero cells was observed at the concentrations of 0.1–1.0 mg/mL. The lethal dose (LC 50 ) of hexane, ethyl acetate and methanol fractions were recorded at (0.30 ± 0.07), (0.52 ± 0.05) and (0.22 ± 0.04) mg/mL, respectively. Some of the compounds identified from ME were caryophyllene (25.21%), neophytadiene (11.90%), α-humulene (7.67%), phytol (4.40%), ethyl ester hexadecanoic acid (4.04%) and nerolidol (2.83%) which were known to have various antimicrobial activities. Conclusions Methanol fraction of M. barteri is a potent and safe antimicrobial and antifungal alternative which can be useful in the search for new antimicrobial drugs. The study also confirmed the orthodox usage of M. barteri in combating infectious diseases.
- Subjects
CELL-mediated cytotoxicity; ANTI-infective agents; MACARANGA; PLANT extracts; MEDICATION safety; GAS chromatography/Mass spectrometry (GC-MS)
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2017, Vol 7, Issue 7, p654
- ISSN
2221-1691
- Publication type
Article
- DOI
10.1016/j.apjtb.2017.06.014