We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.
- Authors
Zimmermann, Jakob; Hübschmann, Thomas; Schattenberg, Florian; Schumann, Joachim; Durek, Pawel; Riedel, René; Friedrich, Marie; Glauben, Rainer; Siegmund, Britta; Radbruch, Andreas; Müller, Susann; Chang, Hyun‐Dong
- Abstract
Using high‐resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single‐cell level.
- Publication
European Journal of Immunology, 2016, Vol 46, Issue 5, p1300
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.201646297