We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
GENETIC CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF BACILLUS ANTHRACIS STERNE STRAIN BY 16S RRNA GENE SEQUENCING.
- Authors
Khan, A. R.; Tahir, M. S.; Naz, S.; Chughtai, S. A.; Khan, M. N.; Raana, W.
- Abstract
Anthrax, caused by Bacillus anthracis, is a fatal disease and all the warm blooded animals are susceptible to this disease. Many livestock populated areas in Pakistan are endemic for Anthrax (Thappa and Karthikeyan 2001), therefore the livestock population is annually vaccinated against Anthrax. Veterinary Research Institute (VRI) has been producing Anthrax Spore Vaccine (ASV) for a couple of decades. The current study was designed for molecular characterization of the microorganism used for vaccine production (Bacillus anthracis Sterne strain) and its phylogenetic relationship with related strains and Bacillus species. For this purpose, 16S rRNA gene was amplified and sequenced and was found 100% genetically similar to the reference sequence with respect to 16S rRNA gene. Phylogenetic analysis revealed that sequenced strain showed a close phylogenecity with Bacillus anthracis strain 1144 and Bacillus anthracis strain V77-NP-1R. Moreover, the organism was found genetically closer to Bacillus cereus than to Bacillus mycoides and Bacillus thuringiensis with respect to 16S rRNA gene sequence.
- Subjects
BACILLUS anthracis; LIVESTOCK diseases; BACTERIA phylogeny; RNA sequencing; GENE amplification; THERAPEUTICS; BACTERIA
- Publication
Pakistan Journal of Science, 2016, Vol 68, Issue 1, p12
- ISSN
0030-9877
- Publication type
Article