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- Title
IAR4 mutation enhances cadmium toxicity by disturbing auxin homeostasis in Arabidopsis thaliana.
- Authors
Chen, Jie; Huang, Shao Bai; Wang, Xue; Huang, LiZhen; Gao, Cheng; Huang, Xin-Yuan; Zhao, Fang-Jie
- Abstract
Cadmium (Cd) is highly toxic to plants, but the targets and modes of toxicity remain unclear. We isolated a Cd-hypersensitive mutant of Arabidopsis thaliana , Cd-induced short root 2 (cdsr2), in the background of the phytochelatin synthase-defective mutant cad1-3. Both cdsr2 and cdsr2 cad1-3 displayed shorter roots and were more sensitive to Cd than their respective wild type. Using genomic resequencing and complementation, IAR4 was identified as the causal gene, which encodes a putative mitochondrial pyruvate dehydrogenase E1 α subunit. cdsr2 showed decreased pyruvate dehydrogenase activity and NADH content, but markedly increased concentrations of pyruvate and alanine in roots. Both Cd stress and IAR4 mutation decreased auxin level in the root tips, and the effect was additive. A higher growth temperature rescued the phenotypes in cdsr2. Exogenous alanine inhibited root growth and decreased auxin level in the wild type. Cadmium stress suppressed the expression of genes involved in auxin biosynthesis, hydrolysis of auxin-conjugates and auxin polar transport. Our results suggest that auxin homeostasis is a key target of Cd toxicity, which is aggravated by IAR4 mutation due to decreased pyruvate dehydrogenase activity. Decreased auxin level in cdsr2 is likely caused by increased auxin-alanine conjugation and decreased energy status in roots.
- Subjects
AUXIN; CADMIUM; NADH dehydrogenase; ROOT growth; POISONOUS plants; HOMEOSTASIS; ARABIDOPSIS; ARABIDOPSIS thaliana
- Publication
Journal of Experimental Botany, 2024, Vol 75, Issue 1, p438
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erad366