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- Title
Cryopreservation and in vitro conservation of virus free plant genetic resources and species tolerant to biotic and abiotic stresses.
- Authors
Abouzedan, T.; Alsameen, M.; AL-Bisky, F.; Maarri, K. Al
- Abstract
Biotechnology plays a major role not only in the production of plants tolerant to biotic and abiotic stresses, but also in the conservation of plant genetic resources to establish Gene Bank. In this investigation, cryopreservation by encapsulation -dehydration technique of Grape virus-free plants and Iris aurantica has been established for the first time in Syria. Factors affecting different stages of cryopreservation (pre-freezing, capsulation, dehydration, and freezing stage) have been studied to improve the survival rate of virus-free plants after re-freezing. The use of cryopreservation technology is the only way to ensure the long-term preservation of virus-free grapes and Iris by storing shoot apex in liquid nitrogen (-196°C) and storing them at very low temperatures, such as the freezing point of CO2 (-80°C). In vitro preservation was conducted to develop a technology for short-term conservation and relieve of growth and increase the period between transfers of Iris aurantica. In this study, the best osmotic agents for in vitro conservation was sucrose compared to mannitol and sorbitol, and the best medium concentration was 1/10 MS. The cultured stored at (3°C) gave the highest survival (93.33%) and lengthened the time period between transfers up to 6 months.
- Subjects
SYRIA; PLANT germplasm; PLANT viruses; ABIOTIC stress; GERMPLASM; FREEZING points; SHOOT apexes; SOIL freezing
- Publication
Journal of Agricultural & Marine Sciences (JAMS), 2023, Vol 28, Issue 2, p61
- ISSN
2410-1060
- Publication type
Article