We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG.
- Authors
Chen, Guanyang; Li, Zhehong; Sang, Qing; Wang, Liang; Wuyun, Qiqige; Wang, Zheng; Chen, Weijian; Yu, Chengyuan; Lian, Dongbo; Zhang, Nengwei
- Abstract
Objective: This study intends to establish a nomogram based on inflammatory response-related methylation sites in intraoperative visceral adipose tissue (VAT) to predict excess weight loss (EWL)% at one-year after LSG. Methods: Based on EWL% at one-year after LSG, patients were divided into two groups: the satisfied group (group-A, EWL%≥ 50%) and the unsatisfied group (group-B, EWL%< 50%). Next, we defined genes corresponding to the methylation sites in the 850 K methylation microarray as methylation-related genes (MRGs). We then took the intersection of MRGs and inflammatory response-related genes. After that, inflammatory response-related methylation sites were identified based on overlapping genes. Moreover, difference analysis was carried out to obtain inflammatory response-related differentially methylated sites (IRRDMSs) between group-A and group-B. LASSO analysis was used to identify the hub methylation sites. Finally, we developed a nomogram based on the hub methylation sites. Results: There were 26 patients in the study, with 13 in group-A and 13 in group-B. After data filtering and difference analysis, 200 IRRDMSs were identified (143 hypermethylated sites and 57 hypomethylated sites). Then, we identified three hub methylation sites (cg03610073, cg03208951, and cg18746357) by LASSO analysis and built a predictive nomogram (Area under the curve=0.953). Conclusion: The predictive nomogram based on three inflammatory-related methylation sites (cg03610073, cg03208951, and cg18746357) in intraoperative visceral adipose tissue can predict one-year EWL% after LSG effectively.
- Subjects
METHYLATION; ADIPOSE tissues; NOMOGRAPHY (Mathematics); WEIGHT loss; DNA methylation; SLEEVE gastrectomy
- Publication
Diabetes, Metabolic Syndrome & Obesity: Targets & Therapy, 2023, Vol 16, p1335
- ISSN
1178-7007
- Publication type
Article
- DOI
10.2147/DMSO.S402687