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- Title
Enrichment and Isolation of Surfactin-degrading Bacteria.
- Authors
Hiroshi Habe; Yuya Sato; Toshiaki Taira; Tomohiro Imura
- Abstract
A total of 100 environmental samples were investigated for their ability to degrade 1 g/L surfactin as a substrate. Among them, two enrichment cultures, which exhibited microbial growth as well as surfactin degradation, were selected and further investigated. After several successive cultivations, nanopore sequencing of full-length 16S rRNA genes with MinIONTM was used to analyze the bacterial species in the enrichment cultures. Variovorax spp., Caulobacter spp., Sphingopyxis spp., and Pseudomonas spp. were found to be dominant in these surfactin-degrading mixed cultures. Finally, one strain of Pseudomonas putida was isolated as a surfactin-degrading bacterium. This strain degraded 1 g/L surfactin below a detectable level within 14 days, and C13 surfactin was degraded faster than C15 surfactin.
- Subjects
SURFACTIN; RIBOSOMAL RNA genetics; BACTERIAL cultures; PSEUDOMONAS putida; CAULOBACTER
- Publication
Journal of Oleo Science, 2021, Vol 70, Issue 4, p581
- ISSN
1345-8957
- Publication type
Article
- DOI
10.5650/jos.ess20331