We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Microalgal crop protection by allelopathic control.
- Authors
RHEAY, HANAH
- Abstract
Mass cultivation is an important process that still needs significant improvement to make algae a viable source of renewable energy. The allelopathic interaction in polycultures of Chlorella sorokiniana LANL1228 and Coelastrella sp. was investigated to determine if this phenomenon could be utilized as a crop protection strategy. The culture exudate was collected, purified, and used to introduce allelopathic compounds into 96-well microplates for cultivation of algae. Exudate bioassay experiments were performed with three exudates (Chlorella 1228 exudate, Coelastrella exudate, 50/50 polyculture exudate) each at five concentration treatments. Fractionation of the exudates was performed using solid-phase extraction in methanol. The fractions were used to culture bioassays of C. sorokiniana LANL1228 in 96-well microplates. Based on bioassay activity, fractions were classified as either active or non-active, and analyzed using Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) to identify presence of allelopathic agents. Based on experimental results, Coelastrella is identified as a superior competitor and the interaction is classified as a negative allelopathic interaction. Allelopathic compounds are only present in exudates procured from cultures with Coelastrella under direct competition with Chlorella. The exodume of algal interactions as shown by FT-ICR MS is complex and chemically diverse. Two fractionation regions of allelopathic activity suggest there are two potential compounds or compound classes to be elucidated. The observed allelochemical interaction likely includes long-chain fatty acids, wax esters, or another lipid type compounds. Selective use and design of polycultures is a likely solution to prevent negative allelopathic interactions and will be investigated.
- Subjects
MICROALGAE; ALLELOPATHIC agents; PLANT protection; ION cyclotron resonance spectrometry; FATTY acids; PLANT exudates; WAX esters
- Publication
New Mexico Journal of Science, 2016, Vol 50, Issue 1, p83
- ISSN
0270-3017
- Publication type
Article