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- Title
Chemical Profile and Assessment of Antioxidant, Anti-Inflammatory, and Antiproliferative Effects of Ammodaucus leucotrichus Coss. & Dur. Fruit Extracts on Human Breast Cancer.
- Authors
Belbachir, Fatima Zohra; Ramdani, Lamia Hamdan; Decombat, Caroline; Gainche, Mael; Chalard, Pierre; Djabbou, Nassim; Taibi, Nadia; Bachari, Khaldoun; Yahia, Kamel Dali; Delort, Laetitia; Caldefie-Chezet, Florence
- Abstract
Objective: In this study, we analyzed and investigated the antioxidant, anti-inflammatory, and antitumoral properties of methanolic (Met), dichloromethane (Dic), ethyl acetate (Ac), and diethyl ether (Et) fruit extracts of Ammodaucus leucotrichus on the MCF-7 breast cancer cell line. Methods: The extracts of Ammodaucus leucotrichus fruits, explored in this study, were obtained by cold maceration and analyzed by LC-MS. 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-Carotene and Bovine Serum Albumin (BSA) assays were used to assess antioxidant and anti-inflammatory activities. The antiproliferative and proapoptotic effects of extracts were determined by the Resazurin test and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Results: Phytochemical screening of Ammodaucus leucotrichus fruit extracts revealed the presence of flavonoids (luteolin and cynaroside), iridoid derivatives, phenolic acids, and identified Rhodojaponin--a major cytoxic compound. The DPPH and BSA assays revealed the most potent radical-scavenging activity (IC50 =0.16 mg/mL) and anti-inflammatory effect (IC50 =35.87 μg/mL) in Met extract. Met, Dic, Ac, and Et extracts, at 100 μg/mL, exhibited significant antiproliferative activity against MCF-7 reaching inhibition percentage of 34%, 31%, 28%, and 13%, respectively. qRT-PCR analysis was marked by the inhibition of proliferative and inflammatory markers Proliferating Cell Nuclear Antigen (PCNA) and IL-6 and the up-regulation of antioxidant and proapoptotic genes Nrf2, Bax, and Caspase 3 in Met and Dic extracts, without significant changes to anti-apoptotic gene Bcl-2 expression. Conclusion: The study revealed the potential therapeutic effects of Ammodaucus leucotrichus fruit extracts, suggesting they could be used as an adjuvant to breast cancer therapies.
- Subjects
FRUIT extracts; BREAST cancer; PROLIFERATING cell nuclear antigen; BCL-2 genes; POLYMERASE chain reaction
- Publication
Natural Product Communications, 2024, Vol 19, Issue 3, p1
- ISSN
1934-578X
- Publication type
Article
- DOI
10.1177/1934578X241239827