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- Title
Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration.
- Authors
Kawaguchi, Hiroyuki; Ogawa, Tetsuji; Kurihara, Hidemi; Nanci, Antonio
- Abstract
The interface between denuded dentin and regenerative periodontal tissue was investigated in a rat alveolar bone defect model using morphological and immunocytochemical approaches. The dentin surface was surgically exposed along the palatal roots of maxillary first molar. At 3 weeks post treatment, animals were perfused and treated regions from decalcified mandibles were embedded in Epon for ultrastructural studies or LR White for post-embedding immunogold labeling. Thin tissue sections were incubated with antibodies against noncollagenous matrix (osteopontin, bone sialoprotein, osteocalcin and fibronectin) and plasma (α2HS-glycoprotein and albumin) proteins. While in some cases, regenerative events took place directly on the denuded dentin surface, the interface between the denuded dentin and regenerating periodontal tissue was frequently characterized by the presence of an interfacial zone. This zone sometimes showed an electron-dense, current line-like, planar accumulation of organic material immunoreactive for osteopontin and bone sialoprotein. Immunolabeling for osteocalein and α2HS-glycoprotein was moderate and diffuse throughout the interfacial zone, whereas labeling with antibodies to albumin and fibronectin resulted in a weak reaction. It is concluded that accumulation of bone sialoprotein and osteopontin is a primary even during the formation of regenerative cementum onto denuded root surfaces.
- Subjects
DENTAL pulp; DENTIN; EXTRACELLULAR matrix proteins; IMMUNOGLOBULINS; PERIODONTICS; DENTISTRY; PERIODONTAL disease
- Publication
Journal of Periodontal Research, 2001, Vol 36, Issue 4, p205
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1034/j.1600-0765.2001.036004205.x