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- Title
Breeding Based Remobilization of Tol2 Transposon in <i>Xenopus tropicalis</i>.
- Authors
Lane, Maura A.; Kimber, Megan; Khokha, Mustafa K.
- Abstract
Xenopus is a powerful model for studying a diverse array of biological processes. However, despite multiple methods for transgenesis, relatively few transgenic reporter lines are available and commonly used. Previous work has demonstrated that transposon based strategies are effective for generating transgenic lines in both invertebrate and vertebrate systems. Here we show that the Tol2 transposon can be remobilized in the genome of X. tropicalis and passed through the germline via a simple breeding strategy of crossing transposase expressing and transposon lines. This remobilization system provides another tool to exploit transgenesis and opens new opportunities for gene trap and enhancer trap strategies.
- Subjects
XENOPUS; VERTEBRATES; ANIMAL breeding; TRANSPOSONS; TRANSPOSASE genetics; GERM cells
- Publication
PLoS ONE, 2013, Vol 8, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0076807