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- Title
The Positron Emission Tomography Tracer 3’-Deoxy-3’-[<sup>18</sup>F]Fluorothymidine ([<sup>18</sup>F]FLT) Is Not Suitable to Detect Tissue Proliferation Induced by Systemic Yersinia enterocolitica Infection in Mice.
- Authors
Wiehr, Stefan; Rolle, Anna-Maria; Warnke, Philipp; Kohlhofer, Ursula; Quintanilla-Martinez, Leticia; Reischl, Gerald; Autenrieth, Ingo B.; Pichler, Bernd J.; Autenrieth, Stella E.
- Abstract
Most frequently, gram-negative bacterial infections in humans are caused by Enterobacteriaceae and remain a major challenge in medical diagnostics. We non-invasively imaged moderate and severe systemic Yersinia enterocolitica infections in mice using the positron emission tomography (PET) tracer 3’-deoxy-3’-[18F]fluorothymidine ([18F]FLT), which is a marker of proliferation, and compared the in vivo results to the ex vivo biodistributions, bacterial loads, and histologies of the corresponding organs. Y. enterocolitica infection is detectable with histology using H&E staining and immunohistochemistry for Ki 67. [18F]FLT revealed only background uptake in the spleen, which is the main manifestation site of systemic Y. enterocolitica-infected mice. The uptake was independent of the infection dose. Antibody-based thymidine kinase 1 (Tk-1) staining confirmed the negative [18F]FLT-PET data. Histological alterations of spleen tissue, observed via Ki 67-antibody-based staining, can not be detected by [18F]FLT-PET in this model. Thus, the proliferation marker [18F]FLT is not a suitable tracer for the diagnosis of systemic Y. enterocolitica infection in the C57BL/6 animal model of yersiniosis.
- Subjects
YERSINIA enterocolitica infections; POSITRON emission tomography; THYMIDINE; ENTEROBACTERIACEAE diseases; SEVERITY of illness index; LABORATORY mice; DIAGNOSIS
- Publication
PLoS ONE, 2016, Vol 11, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0164163