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- Title
Development and design of a ‘ready-to-use’ reaction plate for a PCR-based simultaneous detection of animal species used in foods.
- Authors
Laube, Ines; Zagon, Jutta; Spiegelberg, Almuth; Butschke, Andreas; Kroh, Lothar W.; Broll, Hermann
- Abstract
Different TaqManTM-polymerase chain reaction systems have been developed, which allow the detection of even minute amounts of beef, pork, lamb, goat, chicken, turkey and duck in processed foods. The species-specific systems are able to amplify DNA regions with no more than 108 bp in size (exception: duck, 212 bp) located on the single-copy genes cyclic guanosine monophosphate (cyclic GMP) phosphodiesterase, ryanodine receptor and interleukin-2 precursor. The parallel detection of the common ingredient ‘meat’ produced from mammals and poultry was based on the amplification of a region of the myostatin gene. The limit of detection was determined to be ten genome copies for each system. The relative SD under repeatability condition was below 30%. In addition, a ‘ready-to-use’ reaction plate has been developed, which makes it possible to investigate the presence of the seven animal species in parallel after a single real-time run.
- Subjects
DIAGNOSTIC use of polymerase chain reaction; FOOD inspection; GENE amplification; CYCLIC guanylic acid; RYANODINE receptors; INTERLEUKIN-2; BEEF cattle; PROCESSED foods
- Publication
International Journal of Food Science & Technology, 2007, Vol 42, Issue 1, p9
- ISSN
0950-5423
- Publication type
Article
- DOI
10.1111/j.1365-2621.2006.01154.x