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- Title
Identification of N-acyl- l-homoserine lactones produced by non-pigmented Chromobacterium aquaticum CC-SEYA-1 and pigmented Chromobacterium subtsugae PRAA4-1.
- Authors
Rekha, P.; Young, Chiu-Chung; Arun, A.
- Abstract
Many members of the genus Chromobacterium produce violacein, a characteristic purple pigment which is induced by small diffusible N-acyl homoserine lactones (AHL) quorum-sensing molecules. In this study, the production of AHL of the non-pigmented C. aquaticum CC-SEYA-1 and the pigmented C. subtsugae PRAA4-1 were determined by using a CV026 biosensor assay. The profile of AHL was identified from the extracts of stationary phase cultures using gas chromatography-mass spectroscopy (GC-MS) and thin layer chromatography (TLC). CV026 biosensor assay revealed that both the non-pigmented C. aquaticum CC-SEYA-1 and the pigmented C. subtsugae PRAA4-1 produced AHL molecules, which were identified, respectively, as N-octanoyl homoserine lactone (OHL) [also known as C-8 homoserine lactone (C8-HSL)] and N-hexanoyl homoserine lactone (HHL) [also known as C-6 homoserine lactone (C6-HSL)]. The pigment produced by C. subtsugae PRAA4-1 was similar to that of Chromobacterium violaceum ATCC12472 but no characteristic visible spectral peaks of the pigment were observed in the extracts of C. aquaticum CC-SEYA-1. In addition, C. aquaticum CC-SEYA-1 and C. subtsugae PRAA4-1 showed hemolytic activities.
- Publication
3 Biotech, 2011, Vol 1, Issue 4, p239
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-011-0029-1