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- Title
Mouse/Human T--Cell Hybrids Rosetting with Sheep Erythrocytes.
- Authors
Suomalainen, H. A.; Goldsby, R. A.; Osborne, B. A.; Schröder, J.
- Abstract
Hybrid cells have been recovered from selective culture medium after fusion of concanavalin-A-activated human lymphocytes with an AKR mouse thymoma (BW 5147). AFter 6 months of culture twenty-seven out of forty-nine clones still contained human chromosomes. Human chromosome 6 was present in 89% of these clones, and human X in 70%. Clones from one hybrid line contained several human chromosomes. In twelve of the clones carrying human chromosomes, the rosetting with sheep erythrocytes (SRBC) was 3 times as high as in the BW 5147 cell line, All these clones carried the human chromosome 6, and eight clones contained the human X chromosome us well. In some of these clones (25%) chromosome 6 was the only human one present. In the two clones in which human chromosome 6 was completely missing, the rosetting with SRBC was at the level of the BW line. We therefore suggest that genes on human chromosome 6 are responsible for rosetting with SRBC.
- Subjects
T cells; LYMPHOCYTES; ERYTHROCYTES; X chromosome; HUMAN chromosomes; MOLECULAR cloning; SHEEP as laboratory animals
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 2, p163
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00222.x