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- Title
The Polyclonal B-Cell-Activating Property of Protein A Is Not Due to Its Interaction with the Fc Part of Immunoglobulin Receptors.
- Authors
Möller, G.; Landwall, P.
- Abstract
Protein A from Staphylococcus aureus bacteria was found to be a B-cell mitogen and a potent polyclonal B-cell activator (PBA) of antibody synthesis for murine lymphocytes in the absence of macrophages or T lymphocytes. It did not activate T lymphocytes. We investigated whether the interaction between protein A and the Fc part of Ig molecules was responsible for the PBA activity. Protein A failed to induce IgG synthesis in spleen cells from normal mice, even though it binds effectively to IgG molecules. Lymphocytes treated with anti-immunoglobulin antisera followed by protein A were not activated to a larger extent than non-pretreated cells, although only the former cells bound protein A. Finally, direct attempts to suppress the PBA property of protein A by blocking the Fc binding ability with serum or human gamma globulin failed. Wt concluded that protein A possesses two separate biologriral properties, namely to interact with the Fc receptor on Ig molecules and to act as a PBA. Ami these properties are carried out by different parts of the molecule. These findings confirm previous failures to find an active role of the Ig receptors on B lymphocytes in die triggering process.
- Subjects
IMMUNOGLOBULIN G; STAPHYLOCOCCUS aureus; STAPHYLOCOCCUS aureus infections; KILLER cells; BLOOD proteins; LYMPHOCYTES
- Publication
Scandinavian Journal of Immunology, 1977, Vol 6, Issue 4, p357
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1977.tb00405.x