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- Title
Demonstration of In vivo Expression of a Hypothetical Open Reading Frame (ORF-14) Encoded by the RD1 Region of Mycobacterium tuberculosis.
- Authors
Amoudy, H. A.; Ahmad, S.; Thole, J. E.; Mustafa, A. S.
- Abstract
Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.
- Subjects
MYCOBACTERIUM tuberculosis; ANTIGENS; GENOMICS; MYCOBACTERIUM bovis; ESCHERICHIA coli; POLYACRYLAMIDE gel electrophoresis
- Publication
Scandinavian Journal of Immunology, 2007, Vol 66, Issue 4, p422
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2007.01961.x